How can I avoid well-to-well variability after feeding cardiomyocyte cultures in multiwell plates?

To prevent well-to-well variability after feeding iCell® Cardiomyocytes, we recommend:

• Keeping the wells wet: do not allow the wells to dry between medium removal and replacement.
• Feeding no more than three rows or columns of the plate at a time.
• Avoiding contact with the bottom of the plate (touching or scratching).
• Tilting the culture plate 30 to 45 degrees to remove any medium from the edge of the plate.
• Using a manual multichannel pipette to remove medium; do not use an aspirator, which can disrupt the monolayer.
• Setting low flow rates for additions and exchanges on robotic liquid handlers.