Which extracellular matrix (ECM) do you recommend for modeling hypertrophy with cardiomyocytes?

For modeling cardiac hypertrophy with iCell® Cardiomyocytes, we recommend you use fibronectin as the ECM. Prepare the fibronectin according to the manufacturer’s instructions. Coat plates with the fibronectin at a concentration of 5 µg/ml by adding 100 µl/well for 96-well plates and then incubate the plate at 37°C for 1 hour.

For details about other ECMs compatible with standard assays, see the related solution, below.

References:

  1. Application Protocols: Modeling Cardiac Hypertrophy
  2. Carlson et al (2013) Phenotypic screening, with human iPS cell-derived cardiomyocytes: HTS-compatible assays for interrogating cardiac hypertrophy.