Do you have a patch clamp protocol for cardiomyocytes?

Our Application Protocol demonstrates the utility of and consistency in recording action potentials from iCell® Cardiomyocytes using a manual perforated patch clamp method. This protocol provides instructions for culturing the cardiomyocytes, preparing the perforated patch clamp, identifying suitable cardiomyocytes for patch clamp, filling the recording pipette, forming the gigaohm (GΩ) seal, recording action potentials, preparing saline solutions, managing voltage protocol settings, and washing the glassware.

References:

  1. iCell Cardiomyocytes User's Guide
  2. Application Protocol: Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp