How are iCell DopaNeurons media prepared, supplemented, and stored?

The media for iCell® DopaNeurons includes iCell DopaNeurons Maintenance Medium, Medium Supplement, and Nervous System Supplement. Note that the two supplements contain different components and both are required to prepare the Complete Medium recommended in our User's Guide. Medium and supplements are covered under warranty for 1 year from the date of shipment when stored at 4°C and –20°C, respectively. To prepare Complete iCell DopaNeurons Maintenance Medium, thaw the supplements at room temperature prior to use and pipette them into iCell DopaNeurons Maintenance Medium. This Complete Medium is stable for approximately 4 weeks when stored at 4°C.

Important Notes:

  • Do not thaw or refreeze the Medium Supplement or the Nervous System Supplement.
  • Do not freeze Complete Medium.
  • Do not warm Complete Medium to 37°C

Reference:

  1. iCell DopaNeurons User's Guide