What molecular markers are used to characterize iCell DopaNeurons?

The iCell® DopaNeurons are characterized as 95% pure for MAP2/FoxA2/Lmx2 (midbrain identity) and 80% TH positive (dopaminergic), and cells cultured and assayed in complete that iCell DopaNeuron Maintenance Medium should express markers immediately. As shown through flow cytometry, the projections extend the TH staining will become more prominent, but will be reduced over time if a different medium is used.

From the ICC below, it appears that iCell DopaNeurons are a mixed population of A9 and A10 subtype dopaminergic neurons.

Abbreviation Description Localization
MAP2 microtubule-associated protein 2
FoxA2 forkhead box protein A2 (hepatocyte nuclear factor 3-beta or HNF-3B)
Lmx2 LIM homeobox transcription factor 2
Lmx1 LIM homeobox transcription factor 1
TH tyrosine hydroxylase
TUJ1 β-III tubulin
Calbindin (A10 marker)
GIRK2 G-protein-activated inwardly rectifying potassium channel 2 (A9 marker, but some papers indicate that can be also expressed in A10 subtype neurons)

For more information about characterization and morphology, see Solution 00000176A.

References:

  1. iCell DopaNeurons User’s Guide
  2. iCell DopaNeurons Datasheet
  3. Application Protocol: Immunofluorescent Labeling