What molecular markers are used to characterize iCell DopaNeurons?
The iCell® DopaNeurons are characterized as 95% pure for MAP2/FoxA2/Lmx2 (midbrain identity) and 80% TH positive (dopaminergic), and cells cultured and assayed in complete that iCell DopaNeuron Maintenance Medium should express markers immediately. As shown through flow cytometry, the projections extend the TH staining will become more prominent, but will be reduced over time if a different medium is used.
From the ICC below, it appears that iCell DopaNeurons are a mixed population of A9 and A10 subtype dopaminergic neurons.
|MAP2||microtubule-associated protein 2|
|FoxA2||forkhead box protein A2 (hepatocyte nuclear factor 3-beta or HNF-3B)|
|Lmx2||LIM homeobox transcription factor 2|
|Lmx1||LIM homeobox transcription factor 1|
|GIRK2||G-protein-activated inwardly rectifying potassium channel 2 (A9 marker, but some papers indicate that can be also expressed in A10 subtype neurons)|
For more information about characterization and morphology, see Solution 00000176A.