Nanion Cardiac Safety Symposium 2021

Don't miss this 2-day interactive online event:

Current Challenges in Safety Pharmacology, Cardiac Physiologyin silico Modelling, and Best Practices and Evolution of Safety Pharmacology Methods

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Date and time:
Start: Wednesday, May 19,  1pm CEST, 7am EDT
End: Thursday, May 20, 1pm CEST, 7am EDT

FUJIFILM Cellular Dynamics Talk

Speaker: Ravi Vaidyanathan, PhD, Product Manager - Cardiac, FUJIFILM Cellular Dynamics, Inc.

Time: Wednesday, May 19,  8:35am CST (3:35pm CEST).

Title: Predictive and Physiologically Relevant 3D Tri-culture Cardiac Microtissue with iCell® Cardiomyocytes, Endothelial Cells and Primary Cardiac Fibroblast

Abstract: Human iPSC-derived cardiomyocytes (iPSC-CM) are a well-established model for cardiac toxicity testing. The adult human ventricle is comprised of myocytes, fibroblasts, endothelial cells, and other supporting cell types. Although cardiomyocytes account for 75% of myocardial tissue volume, they constitute only 50% of cell numbers. Recent publications have shown that 3D co-culture cardiac microtissues, containing iCell® Cardiomyocytes, endothelial cells, and cardiac fibroblasts have improved predictivity for inotropic compounds compared to cultures of iPSC-CM. Implementation of this 3D platform, however, is challenging due to its technical complexity. Here we present a simple workflow for culturing 3D tri-culture cardiac microtissues and show resulting functional data. These findings suggest that 3D tri-culture cardiac microtissues can be easily assembled from commercially available cryopreserved cells and cultured with off-the-shelf media. The 3D tri-culture cardiac microtissues retain their cell composition and achieve a superior cardiomyocyte response, resulting in a more predictive and physiologically relevant model.

Event Details:

Event Date: 2021-05-19 To 2021-05-20

Organiser: Nanion Technologies