Don't miss this 2-day interactive online event:
Current Challenges in Safety Pharmacology, Cardiac Physiology, in silico Modelling, and Best Practices and Evolution of Safety Pharmacology Methods
Date and time:
Start: Wednesday, May 19, 1pm CEST, 7am EDT
End: Thursday, May 20, 1pm CEST, 7am EDT
FUJIFILM Cellular Dynamics Talk
Speaker: Ravi Vaidyanathan, PhD, Product Manager - Cardiac, FUJIFILM Cellular Dynamics, Inc.
Time: Wednesday, May 19, 8:35am CST (3:35pm CEST).
Title: Predictive and Physiologically Relevant 3D Tri-culture Cardiac Microtissue with iCell® Cardiomyocytes, Endothelial Cells and Primary Cardiac Fibroblast
Abstract: Human iPSC-derived cardiomyocytes (iPSC-CM) are a well-established model for cardiac toxicity testing. The adult human ventricle is comprised of myocytes, fibroblasts, endothelial cells, and other supporting cell types. Although cardiomyocytes account for 75% of myocardial tissue volume, they constitute only 50% of cell numbers. Recent publications have shown that 3D co-culture cardiac microtissues, containing iCell® Cardiomyocytes, endothelial cells, and cardiac fibroblasts have improved predictivity for inotropic compounds compared to cultures of iPSC-CM. Implementation of this 3D platform, however, is challenging due to its technical complexity. Here we present a simple workflow for culturing 3D tri-culture cardiac microtissues and show resulting functional data. These findings suggest that 3D tri-culture cardiac microtissues can be easily assembled from commercially available cryopreserved cells and cultured with off-the-shelf media. The 3D tri-culture cardiac microtissues retain their cell composition and achieve a superior cardiomyocyte response, resulting in a more predictive and physiologically relevant model.