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iCell Astrocytes Kit, 01434
$800.00
Product Overview
Astrocytes are specialized glial cells and the most abundant cell type in the central nervous system (CNS). Astrocytes have historically been regarded as support cells for neural tissue, with reactive astrocytes serving as markers for damaged or diseased tissue. However, new studies reveal that astrocytes play an essential and complex role in the maintenance of a healthy CNS and in the onset and development of CNS disease.
CDI’s iCell® Astrocytes are human iPS cell-derived astrocytes. They provide a readily accessible, consistent, and biologically relevant source of astrocytes for the study of synaptic transmission and plasticity in normal CNS function and disease progression. Benefits include:
- >95% pure population of human astrocytes
- Expression of relevant astrocyte markers (e.g. S100β and GFAP)
- Exhibition of cytokine-mediated inflammatory responses
- Limited proliferative capacity and long-term viability
- Ability to co-culture with human neurons
Enhance the Relevance of Your Neuroscience Models
Assayed as a pure culture of astrocytes or as a co-culture with other iCell neural cell types, iCell Astrocytes provide multiple layers of biologic complexity to enable the interrogation of the following:Normal CNS Functions
- Synaptic remodeling and pruning
- Neurotransmitter homeostasis
- Neural network communication
- Blood-brain barrier support
- Regulation of CNS blood flow
- CNS metabolism
Disease CNS Functions
- Reactive astrogliosis and CNS inflammation
- Neurodegenerative disease
- Neuropsychiatric disease
- Alexander disease and leukodystrophies
- Multiple sclerosis and autoimmune inflammatory disorders
- Infectious disease
Components:
Catalog #R1092
iCell Astrocytes Kit, 01434, 1 Vial: ≥ 1 x 10^6 cells/vial
- Cryopreserved iCell Astrocytes, 01434
- User’s Guide
- Additional reagents required; see the Quick Guide
Donor Background
Female
Caucasian
Astrocytes
Apparently Healthy Normal
Healthy
Fibroblast
<18
No
Apparently Healthy Normal
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
USER DOCS
Biosafety Document
iCell Astrocytes Biosafety Document
Datasheet
iCell Astrocytes
Quick Guide
iCell Astrocytes
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Germany
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - North America
Performance Data
Morphology, Marker Expression, Cytokine Stimulation, Induction of Morphology Changes and Cytokine Upregulation
A highly pure astrocyte population, iCell Astrocytes show (A) typical morphology and (B) expression of GFAP. Upon stimulation with TNFa, IL-1ß, and IFNɣ, iCell Astrocytes demonstrate (C) changes in cell morphology (stained with GFAP, green) and (D) upregulation of the IL-6 secretion, a pro-inflammatory cytokine.
Figure 1: iCell Astrocytes Exhibit Characteristics of Native Human Astrocytes
Product Highlights
Enhance the Relevance of Your Neuroscience Models
Assayed as a pure culture of astrocytes or as a co-culture with other iCell neural cell types, iCell Astrocytes provide multiple layers of biologic complexity to enable the interrogation of the following: Normal CNS functions
- Synaptic remodeling and pruning
- Neurotransmitter homeostasis
- Neural network communication
- Blood-brain barrier support
- Regulation of CNS blood flow
- CNS metabolism
- Reactive astrogliosis and CNS inflammation
- Neurodegenerative disease
- Neuropsychiatric disease
- Alexander disease and leukodystrophies
- Multiple sclerosis and autoimmune inflammatory disorders
- Infectious disease
- Alexander disease and leukodystrophies
- Multiple sclerosis and autoimmune inflammatory disorders
- Infectious disease
Publications
Three-dimensional Brain-on-chip Model Using Human iPSC-derived GABAergic Neurons and Astrocytes: Butyrylcholinesterase Post-treatment for Acute Malathion Exposure Liu L, Koo Y, Russell T, Gay E, Li Y, Yun Y PLoS One. (2020) 15(3): e0230335. (2020)
Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoate (PFOA) Acutely Affect Human α1β2γ2L GABAA Receptor and Spontaneous Neuronal Network Function in Vitro A Tukker, L Bouwman, R van Kleef, H Hendriks, J Legler, R Westerink. (2020) Sci. Rep. 10, 5311 (2020)
Towards Animal-Free Neurotoxicity Screening: Applicability of hiPSC-derived Neuronal Models for in Vitro Seizure Liability Assessment A Tukker, R van Kleef, F Wijnolts, A De Groot, R Westerink. (2020) ALTEX. 37(1):121-135. (2020)
Excess Glutamate Secreted from Astrocytes Drives Upregulation of P-glycoprotein in Endothelial Cells in Amyotrophic Lateral Sclerosis Mohamed LA, Markandaiah SS, Bonanno S, Pasinelli P, and Trotti D. (2019) Exp Neurol. 316:27-38 (2019)
Human iPS-Derived Astroglia from a Stable Neural Precursor State Show Improved Functionality Compared with Conventional Astrocytic Models Lundin A, Delsing L, Clausen M, Ricchiuto P, Sanchez J, Sabirsh A, Ding M, Synnergren J, Zetterberg H, Brolén G, Hicks R, Herland A, Falk A (2018) Stem Cell Reports 10(3):1030-1045. (2018)