iCell Cardiac Progenitor Cells Kit, 01279

$1,240.00
Catalog # R1093

Product Overview

Cardiac progenitor cells (CPCs) are able to proliferate and have the capacity to differentiate into cardiomyocytes. Thus, these cells are attractive targets in drug development applications for regenerative medicine programs focused on developing myocardial infarction and congestive heart failure treatments. They are also ideal for identifying and helping to avoid drug-mediated cardiac developmental toxicity problems across multiple programs, an application for which scientists require consistent and dependable access to human CPCs.

Derived from human iPS cells, FCDI's iCell® Cardiac Progenitor Cells provide a robust, reproducible, and relevant source of cells to advance drug development and cardiac regenerative medicine programs. Benefits include:

  • KDR+/PDGFR-α+/c-kit– human CPC marker profile
  • bFGF-mediated proliferation
  • Wnt inhibitor-mediated differentiation into cTNT+ cardiomyocytes
  • High-throughput target and phenotypic-based screen compatibility

Components:

Catalog #R1093
iCell Cardiac Progenitor Cells Kit, 01279, 1 Vial: ≥ 5 x 10^6 cells/vial
  • Cryopreserved iCell Cardiac Progenitor Cells, 01279
  • User’s Guide
  • Additional reagents required. Please see User’s Guide.

Donor Background


Male

Caucasian

Cardiac Progenitor Cells

Apparently Healthy Normal

Healthy

Blood

50-59

No

Apparently Healthy Normal

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Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

Differentiation into Cardiac Cells

iCell Cardiac Progenitor Cells differentiated into cardiomyocytes as indicated by the increase in cTNT expression in the induced condition. Acquisition and analysis were performed using a BD Accuri C6 Flow Cytometer (BD Biosciences). iCell Cardiac Progenitor Cells were plated in the presence of XAV939/SB431542 for 2 days and assayed on day 8 post-plating

Figure 1: Flow Cytometry Detection of cTNT Expression in Cardiomyocytes Differentiated from XAV939/SB431542-induced iCell Cardiac Progenitor Cells

Figure 2: Detection and Quantification of bFGF-induced Cardiac Proliferation by High Content Analysis

Induction of Cell Proliferation

In this representative experiment, cell proliferation of iCell Cardiac Progenitor Cells was induced as indicated by the increase in the Hoechst 33342+ /NKX2.5+ /cTNT– cell population in the treatment condition. Acquisition and analysis were performed using the ImageXpress Micro System and MetaXpress Software. iCell Cardiac Progenitor Cells were plated in the presence of bFGF for 2 days and assayed on day 2 post plating.

Product Highlights

iCell Cardiac Progenitor Cells

  • iCell Cardiac Progenitor Cells represent an optimal in vitro test system for interrogating human cardiac regenerative biology due to their human origin, high purity, functional relevance, and ease of use.
  • Suitable for inducing proliferation with basic fibroblast growth factor (bFGF). A detailed protocol is available.
  • Also well suited for induction of proliferation with Wnt inhibitor XAV939 and activin/TGFβ inhibitor SB431542 as assessed by the expression of cardiac troponin T (cTNT) using flow cytometry analysis. A detailed protocol is available.