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Endothelial cells are highly dynamic cells that form the inner lining of blood vessels. In addition to their structural role, endothelial cells regulate the passage of substances between the bloodstream and surrounding tissue. They also secrete mediators that influence vascular hemodynamics, inflammation, cell trafficking, and the remodeling and formation of new blood vessels. Endothelial cell dysfunction is associated with a variety of diseases including atherosclerosis, coronary artery disease, hypertension, and inflammatory disorders.
Derived from human iPS cells, FCDI’s iCell® Endothelial Cells provide a reliable, reproducible, and physiologically relevant source of cells for vascular disease modeling, drug screening, and vascular tissue engineering applications. Benefits include:
Assayed as a pure culture or in co-culture with other differentiated cell types to recapitulate native tissue architecture, iCell Endothelial Cells enable a variety of novel applications:
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Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
The cells have >90% expression of CD105 (endoglin), CD31 (PECAM-1) and CD144 (VE-cad) as determined by flow cytometry analysis.
Figure 1: iCell Endothelial Cells Are a Highly Pure Population
Figure 2: iCell Endothelial Cells Display Expected Markers and Functions
(A) When immunostained for von Willebrand Factor (vWF), the cells showed characteristic Weibel-Palade body staining. (B) In a thick layer of Matrigel, the cells exhibited the capacity to form tubes. (C) To assess the barrier function, the cells were immunostained for the tight junction protein ZO-1.
iCell Endothelial Cells exhibit barrier function activity that can be disrupted by treatment with thrombin and reliably assessed using impedance-based platforms. iCell Endothelial Cells provide an in vitro test system that recapitulates native human endothelium properties and functions while the xCELLigence RTCA Cardio System provides a label-free technology for non-invasive monitoring of cell behavior and viability. The methods and results presented here highlight how to gather relevant data on human endothelial viability and barrier function. Together, iCell Endothelial Cells and impedance-based technologies offer a valuable cell model system for understanding the endothelial barrier characteristics, mechanisms of endothelial barrier dysfunction, and dynamic modulation of the endothelium permeability, enabling a wide range of applications in academic and pharmaceutical research. iCell Endothelial Cells were cultured for 48 hours on the E-Plate in Complete iCell Endothelial Cells Maintenance Medium before the addition of thrombin. (A) The representative Cell Index curves showed the dose-dependent disruption effect of thrombin on the barrier function and the recovery phase. (B) The percentage of Cell Index compared to pre-treatment baseline levels was calculated at the time of thrombin addition, at 9 hours post-treatment, or at 21 hours post-treatment (mean ± SD, n = 2 wells).
Figure 3: Barrier Disruption Effect of Thrombin on iCell Endothelial Cell
iCell Endothelial Cells are amenable to a variety of assays including:
Apolipoprotein E4 Expression Causes Gain of Toxic Function in Isogenic Human Induced Pluripotent Stem Cell-Derived Endothelial Cells Rieker C, Migliavacca E, Vaucher A, Baud G, Marquis J, Charpagne A, Hegde N, Guignard L, McLachlan M, Pooler AM. (2019) Arterioscler Thromb Vasc Biol.39(9):e195-e207. (2019)
Excess Glutamate Secreted from Astrocytes Drives Upregulation of P-glycoprotein in Endothelial Cells in Amyotrophic Lateral Sclerosis Mohamed LA, Markandaiah SS, Bonanno S, Pasinelli P, and Trotti D. (2019) Exp Neurol. 316:27-38 (2019)
Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells Belair DG, Whisler JA, Valdez J, Velazquez J, Molenda JA, Vickerman V, Lewis R, Daigh C, Hansen TD, Mann DA, Thomson JA, Griffith LG, Kamm RD, Schwartz MP, and Murphy WL (2015) Stem Cell Rev Rep 11(3):511-25. doi: 10.1007/s12015-014-9549-5. (2015)
Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds As Biological Templates for Organ Engineering and Transplantation Caralt M, Uzarski JS, Iacob S, Obergfell KP, Berg N, Bijonowski BM, Kiefer KM, Ward HH, Wandinger-Ness A, Miller WM, Zhang ZJ, Abecassis MM, and Wertheim JA (2014) Am J Transplant 15(1):64-75 (2014)
Vascular Tissue Engineering: Building Perfusable Vasculature for Implantation Gui L and Niklason LE (2014) Curr Opin Chem Eng 3:68-74 (2014)
Diabetes Mellitus Aggravates Hemorrhagic Transformation after Ischemic Stroke via Mitochondrial Defects Mishiro K, Imai T, Sugitani S, Kitashoji A, Suzuki Y, Takagi T, Chen H, Oumi Y, Tsuruma K, Shimazawa M, Hara H PLoS One. (2014) 9(8): e103818. (2014)