iCell GABANeurons, 01279

Kit Size

Catalog #: R1011

Cells Only

Catalog #: C1008
Catalog #: C1008

GABANeurons differentiated from human iPS cells, frozen

From
$1,574.00
Catalog # GGBN01279

Product Overview

Traditional neuroscience research tools consist largely of rodent primary cell cultures and animal models, which are labor-intensive, costly, and poorly reflective of native human biology. iCell® GABANeurons represent a relevant human in vitro system for modeling and interrogating complex neurological processes and diseases.

iCell GABANeurons are iPS cell-derived mixed populations of human cerebral cortical neurons that exhibit native electrical and biochemical activity. They overcome limitations of existing models by providing the following:

  • >95% pure population of primarily GABAergic (inhibitory) neurons
  • Rapid formation of neural networks and functional synapses
  • Expression of relevant neurological therapeutic targets and pathways
  • Long-term viability and demonstrated reproducibility
  • Reliable supply in cryopreserved format

More Data, More Publications, More Solutions

There is a broad range of peer-reviewed publications and CDI application protocols and applications notes  to support your research goals including:

  • Alzheimer’s disease research and drug discovery
    Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates
  • Autism disease research
    Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism
  • Infectious disease studies
    Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation
  • Therapeutic potency testing
    High sensitivity, dose-response, and biological responses to Clostridium botulinum neurotoxin (BoNT) aid replacement of the gold-standard mouse bioassay for BoNT potency testing
  • Neurotoxicity assessment
    Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity

Components:

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Our specialists are here to help you find the best product for your application.

Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

Neurite Outgrowth Assay

Panels A and B show iCell GABANeurons, 01434 cultured in a 96-well cell culture plate in absence or presence of 50 ng/ml BDNF, respectively. The neurons were stained with calcein AM (green) and Hoechst 33342 (blue). Panels C and D show the segmentation masks generated from calcein AM and Hoechst 33342 fluorescent signals of panels A and B, respectively. Images were acquired using the ImageXpress Micro System, and segmentation masks were generated using the MetaXpress Software.

Figure 1: Representative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Figure 2: Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Culturing iCell GABANeurons, 01434 in the presence of BDNF for 14 days produced the expected effect of enhancing neurite outgrowth. Data was acquired using the ImageXpress Micro System and quantified using the MetaXpress Software. Data are represented as mean values per number of neuronal cells (mean ± SEM, n = 6 wells).

Relevant Neuronal Markers and High Purity

iCell GABANeurons represent a highly pure population comprised primarily of GABAergic neurons with low levels of nestin (neuronal progenitor marker) as demonstrated by immunocytochemistry. These images show iCell GABANeurons, 01434: (A) ß-III tubulin (neuronal marker, green) and nestin (red), 7 days post-plating; (B) punctate staining pattern for the vesicular glutamate transporter 2 (vGLUT2, red) and vesicular GABA transporter (vGAT, green), 14 days post-plating, indicative of glutamatergic and GABAergic neuronal subtypes, respectively; and (C) gamma-aminobutyric acid (GABA, red) and microtubule associated protein 2 (MAP2, green), 14 days post-plating.

Figure 3: iCell GABANeurons Represent a Highly Pure Population of Human Neurons

Figure 4: iCell GABANeurons Exhibit Typical Neuronal Morphology

Expected  GABAergic Neuron Morphology

These images show iCell GABANeurons, 01434 at days 1, 5, and 19 post-plating. The reanimated GABAergic neurons develop branched networks within 24 hours and remain viable and adherent for an extended period in culture (≥14 days).

Product Highlights

More Data, More Publications, More Solutions

There is a broad range of peer-reviewed publications and CDI application protocols and applications notes to support your research goals including:

  • Alzheimer’s disease research and drug discovery Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates
  • Autism disease research Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism
  • Infectious disease studies Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation
  • Therapeutic potency testing High sensitivity, dose-response, biological responses to Clostridium botulinum neurotoxin
  • Neurotoxicity assessment Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity

Publications