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iCell® Hematopoietic Progenitor Cells 2.0 are highly pure multipotent progenitor cells with the ability to differentiate into blood cell lineages. These cells can be consistently generated from human induced pluripotent stem cells in large-scale quantities, thereby overcoming the high variability and limited quantities associated with primary CD34+ cord blood or peripheral blood cells.
iCell Hematopoietic Progenitor Cells 2.0 were plated in colony-forming unit (CFU) assays using a methylcellulose-based medium. (A) Representative images of colonies are shown. (B) CFU frequencies are shown, including erythroid (BFU-E), granulocyte/macrophage (CFU-M, CFU-G, CFU-GM) and mixed colonies (CFU-GEMM). Data shown as mean ±SEM; n=13.
(A, B) Cells analyzed by flow cytometry at thaw are >90%+ for hematopoietic cell surface markers. (C) Percentage of cells expressing hematopoietic cell surface markers after thaw. Data shown as mean ±SEM; n=13.
iCell Hematopoietic Progenitor Cells 2.0 display typical morphology as freely floating round cells.