iCell Hepatoblasts, 01434

Kit Size

Catalog #: R1096
Catalog #: R1095
Catalog #: R1095

Hepatoblasts differentiated from human iPS cells, frozen

From
$600.00
Catalog # GHPB01434

Product Overview

Hepatoblasts, progenitor cells found in the liver, are defined as endoderm lineage-derived bipotential cells that can differentiate into hepatocytes or cholangiocytes (Figure 1).

iCell Hepatoblasts Figure 1
Figure 1: Hepatoblasts are bipotential progenitor cells that can differentiate into hepatocytes or cholangiocytes. NucBlue (blue), Cytokeratin 7 (red), Alpha-a Antitrypsin (green).

Fujifilm Cellular Dynamics has identified the time point in differentiation where endoderm-specific markers are declining and hepatocyte-specific markers are not significantly expressed (Figure 2). This time point is when our commercially available iCell® Hepatoblasts are cryopreserved.

Upon reanimation, iCell Hepatoblasts are ideal for therapeutic research aimed at liver regeneration via the identification of molecules and factors capable of stimulating progenitor cell proliferation/regeneration. They are also perfect for pro/anti hepatocyte differentiation and pro/anti cholangiocyte differentiation assays. In addition, iCell Hepatoblasts can be combined with iCell Endothelial Cells, iCell Hepatocytes, iCell Macrophages, and iCell Mesenchymal Stem Cells to engineer complex liver models for in vitro and in vivo applications. These cell combinations enable disease model building by providing access to multiple liver cell types from a single donor for co-culture.

iCell Hepatoblasts Hepatocytes & Endoderm Markers
Figure 2: iCell Hepatoblasts represent a time point in differentiation where the cells are no longer positive for endoderm-specific markers, such as CXCR4, and not yet expressing hepatocyte-specific markers, such as A1AT.

iCell Hepatoblasts retain proliferative capacity in vitro, supplying a dynamic cell population that is driven to differentiate (Figure 3). FCDI provides detailed stepwise instructions for thawing, plating, culturing, and differentiating iCell Hepatoblasts into hepatocytes or cholangiocytes.

iCell Hepatoblasts Figure 3
Figure 3: (A) iCell Hepatoblasts demonstrate proliferative potential post-thaw. The cells show evidence of increasing cell number by incorporation of ethidium containing nucleosides into newly synthesized DNA under both hepatocyte- and cholangiocyte-favoring induction conditions. (B, C) iCell Hepatoblasts retain the potential to differentiate into a hepatocyte or cholangiocyte as evidenced by A1AT and CK7 staining. Images were taken 7 days post-thaw following FCDI’s application protocols.Contact FCDI’S Technical Support to learn more about iCell Hepatoblasts and other ways we can further your research through iPS cell technology.

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Performance Data

iCell Hepatoblasts Represent a Population of Bipotential Cells

iCell Hepatoblasts retain the potential to differentiate into both hepatocytes and cholangiocytes. This population of human hepatoblasts is derived from iPS cells using CDI’s proprietary differentiation and purification protocols. These cells provide a reliable source of human hepatoblasts that are suitable for use in

  • preclinical drug discovery
  • toxicity testing
  • other life science research
These images show iCell Hepatoblasts at day 0 post-plating and day 7 post-differentiation into hepatocytes or cholangiocytes. iCell Hepatoblasts can be induced to differentiate toward hepatocyte or cholangiocyte lineages, as demonstrated by flow cytometry, resulting in an increase of alpha-1 antitrypsin or cytokeratin-7 expressing cells, respectively. iCell Hepatoblasts represent a progenitor population upstream in differentiation from the iCell Hepatocytes 2.0. This cell type reflects the point in differentiation from induced pluripotent stem (iPS) cells past endoderm but before being a fully specified hepatocyte. Protocols are available from FCDI to model differentiation into both hepatocytes and cholangiocytes.

Figure 1: iCell Hepatoblasts Represent a Population of Bipotential Cells

Figure 2: Flow Cytometry Analysis of A1AT Expression in Hepatocytes Differentiated from iCell Hepatoblasts

Differentiation into Hepatocytes

In this representative experiment, iCell Hepatoblasts are differentiated into hepatocytes as indicated by the increase in A1AT expression in the induced condition. Acquisition and analysis were performed using a BD Accuri C6 Flow Cytometer (BD Biosciences).

Differentiation into Cholangiocytes

In this representative experiment, iCell Hepatoblasts were differentiated into cholangiocytes as indicated by the increase in CK7 expression in the induced condition (with 20 ng/ml activin A). Acquisition and analysis were performed using a BD Accuri C6 Flow Cytometer (BD Biosciences).

Figure 3: Flow Cytometry Analysis of A1AT and CK7 Expression in Cholangiocytes Differentiated from iCell Hepatoblasts