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The hepatocytes exhibit cobblestone morphology, round nuclei with distinct nucleoli, bi-nucleation (ovals), and formation of bile canaliculi (arrows).*
Figure 1: iCell Hepatocytes 2.0 Show Characteristic Morphology
Figure 2: iCell Hepatocytes 2.0 Exhibit P450 Metabolism Activity
The hepatocytes exhibit robust (A) CYP3A basal activity and (B) CYP3A4 induction activity (response to rifampin measured by P450-Glo™ CYP3A4 Induction/Inhibition Assay, Promega Corp.).
The hepatocytes exhibit expression of (A) alpha-1-antitrypsin and (B) albumin, (C) albumin production (measured by ELISA), (D) glycogen storage (measured by PAS staining), and lipid storage (data not shown).
Figure 3: iCell Hepatocytes 2.0 Exhibit Hepatocyte Protein Expression and Function
Figure 4: Phase Images of 2D and 3D Cultures of iCell Hepatocytes 2.0
(A) Cells are thawed and plated into the collagen-coated cell culture plate to form a 2D confluent monolayer. (B) Cells are then detached and seeded in aULA plate to form 3D spheroids within 2 days. See the application protocol for details.
iCell Hepatocytes 2.0 have been genotyped for 1,936 ADME markers in over 200 genes, including all FDA-validated genes and >90% of the ADME Core markers as defined by the PharmaADME group.
iCell Hepatocytes 2.0 are platable and maintain hepatocyte functions in culture for at least one week.
iCell Hepatocytes are >95% pure, providing biologically relevant and reproducible results.
iCell Hepatocytes 2.0 are terminally differentiated from human iPS cells and exhibit hepatocyte characteristics and functions.