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iCell Microglia, 01279

Kit Size

Catalog #: R1131

Cells Only

Catalog #: C1110
Catalog #:

Microglia differentiated from human iPS cells, frozen

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$1,695.00
Catalog # GMGL01279
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Product Overview

Microglia are immune cells resident in the central nervous system (CNS) responsible for fundamental physiological and pathological processes. Microglia support neuronal homeostasis and facilitate neuronal network formation by synaptic pruning. Surveillant microglia can activate, adapt and respond accordingly to specific stimulus. Microglia dysfunction has been implicated in neurodegeneration afflictions, including Parkinson’s and Alzheimer’s diseases, and neurodevelopmental disorders, such as Rett syndrome. The limited availability and inconsistency of primary human microglia has constrained research and therapeutic progress.

iCell® Microglia are based on technology developed by the Blurton-Jones laboratory (Abud et al. Neuron 2017.) for which FujiFilm Cellular Dynamics Inc. has an exclusive license from the University of California-Irvine.  iCell Microglia overcome the limitations of primary microglial cells by offering a consistent, high-quality microglia derived from human induced pluripotent stem cells, enabling the study of cytokine signaling, synaptic transmission, and plasticity in normal CNS function and during disease progression.

  • Human Relevance 
    iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses, and express relevant microglial markers (TREM2, CX3CR1, TMEM119, P2RY12, IBA1).
  • Reproducible Research
    Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance and reproducible results from every lot of iCell Microglia.
  • Rapid Results
    iCell Microglia are shipped cryopreserved and arrive fully differentiated allowing more experiments to be completed in less time.
  • Disease Models Available
    Engineered disease model lines of iCell Microglia are available for SNCA (A53T), TREM2, and MECP2.

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Our specialists are here to help you find the best product for your application.

Call Us

(877) 320-6688

 

OR

Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

 iCell Microglia are a Highly Pure Microglial Population

Based on published data describing gene expression and signatures of several microglial stages (Abud et al, 2017a>), iCell Microglia represent a valid in vitro model for CNS research. These cells express TREM2, CXCR4, P2RY12, IBA1, and PU.1 microglial markers at over 80% in the overall cell population. Physiologically, iCell Microglia demonstrate critical responses to stimuli including signature cytokine release, and the phagocytosis of bioparticles. Flow cytometry analysis of surface markers shows that iCell Microglia exhibit a characteristic microglia signature.

Figure 1:  iCell Microglia are a Highly Pure Microglial Population

Figure 2: iCell Microglia Secrete Cytokines Upon Stimulation

iCell Microglia Secrete Cytokines Upon Stimulation

iCell Microglia were thawed into 96-well plates and allowed to recover for three days prior to treatment (using lipopolysaccharide (100ng/mL) or IFN gamma (50ng/mL). After 24 hr, cytokine levels in the supernatant were assayed using the Luminex Multiplex Assay. Specific cytokine signatures were observed for each stimulus.

iCell Microglia Phagocytose Bioparticles

Using the IncuCyte® Live-Cell Analysis System (Sartorius AG), Staphylococcus aureus (SA) BioParticles®  Conjugate (ThermoFisher Scientific) were added to iCell Microglia cultures to assess the kinetics of phagocytosis

Figure 3: iCell Microglia Phagocytose Bioparticles

Product Highlights

iCell Microglia Physiology

iCell Microglia are semi-adherent cells amenable to a variety of applications. iCell Microglia express key transcription factors and markers (CX3CR1, IBA1, TMEM119 and P2RY12) that are characteristically found in adult human microglia and are able to phagocytose and secret cytokines upon stimulation. Cell-based Assays Immunofluorescence Phagocytosis Amyloid-beta pHrodo™ Red labeling Figure 1: iCell Microglia Physiology (A) iCell Microglia secrete specific cytokines upon stimulation. (B) iCell Microglia phagocytose BioParticles® in a concentration-dependent manner. Advantages

  • Human relevance: iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses.
  • Reproducible research: Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance from every lot of iCell Microglia (>80% TREM2+, IBA1+, CX3CR1+).
  • Rapid results: iCell Microglia are fully differentiated cells (not precursors) allowing for more experiments in less time.
  • Disease lines available: TREM2, SNCA and MeCP2 engineered mutants and isogenic control.

Publications

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases Abud EM, Ramirez RN, Martinez ES, Healy LM, Nguyen CHH, Newman SA, Yeromin AV, Scarfone VM, Marsh SE, Fimbres C, Caraway CA, Fote GM, Madany AM, Agrawal A, Kayed R, Gylys KH, Cahalan MD, Cummings BJ, Antel JP, Mortazavi A, Carson MJ, Poon WW, Blurton-Jones M (2017) Neuron. 94(2):278-293 (2017)

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