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iCell Microglia Rett Syndrome MECP2, 01279
Product Overview
Rett Syndrome (RTT) is a devastating neurodevelopmental disorder, caused mainly by mutations in the MECP2 gene. MECP2 codes for a 486 amino acid protein that binds methylated DNA, generally downregulating transcription. MECP2 mutations are associated with a range of developmental diseases including RTT, autism, encephalopathy and X-linked intellectual disability. RTT occurs mostly in females since MeCP2 is located on the X chromosome.
The engineered iCell® Microglia, MECP2 line contains an insert directly after Serine 49 that includes stop codons in all 3 reading frames, generating a non-functional truncated protein before the methyl-CpG-binding domain resulting in the protein being p.(Ala50Ter). The MECP2 line was engineered in the otherwise healthy background line, 01279.
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Technical Docs
PROTOCOLS
Application Protocols
Immunofluorescent Labeling
Application Protocols
Labeling Amyloid Beta with pHrodo Red
Application Protocols
Measuring Microglia Phagocytosis: Kinetic Imaging on the IncuCyte
USER DOCS
Biosafety Document
iCell Microglia Biosafety Document
Biosafety Document
iCell Microglia Kit, MECP2, 01279
Datasheet
iCell Microglia
Quick Guide
iCell Microglia Quick Guide
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Germany
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - North America
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet - Asia
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet - North America
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet - North America
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet - North America
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet - North America
Performance Data
Cytokine Response
The cytokine response of stimulated iCell Microglia and the engineered MECP2 line were compared by multiplex assay (Fig. 4). iCell Microglia were plated in microglia maintenance media and allowed to recover for 72 h prior to stimulation with LPS, interferon gamma or both for 24 h. Supernatants were collected and assayed using a Luminex multiplex cytokine assay.
Figure 1: Cytokine Comparison of iCell Microglia and SNCA
Figure 2: Phagocytosis of iCell Microglia and MECP2 Engineered Line
Phagocytosis
In Figure 3, a comparison is shown between iCell Microglia and the engineered MeCP2 line. Phagocytosis of S.aureus BioParticles® was measured over time using an IncuCyte® S3 Live-Cell Analysis System. The engineered microglia exhibit phagocytic activity, but at a substantially reduced level relative to the unengineered line.
Comparative Marker Expression
iCell Microglia were labeled for the presence of cell surface (CD45, CD11b, CD11c, TREM2 and, CD33) and intracellular (P2RY12, TMEM119, CX3CR1, IBA1) antigens by flow cytometry (Fig. 2). The specific staining is compared against matched isotype controls. Expressed marker levels are similar to the control line across the range of markers tested.
Figure 3: Comparison of Marker Expression of MeCP2 and Unmodified iCell Microglia
Figure 4: Western Blot Analysis of MECP2 Mutation
Western Blot Analysis of MECP2
Total cell lysates of iCell GABANeurons from both 01279 and the isogenic harboring the MECP2 mutation were compared by Western blot analysis (Fig. 1). Blot results suggest that the methyl binding domain and downstream elements are not expressed at a detectable level in the MECP2-modified line. Note: MECP2 is ubiquitously expressed, including in neurons. Inquire about availability of iCell GABANeurons harboring the MECP2 mutation. Equivalent loads of total cell protein were compared by Western blot from differentiated iCell GABANeurons were probed with an anti-MeCP2 antibody and normalized to tubulin. (Western blot data courtesy Vanderbilt University)
Product Highlights
Schematic of the MECP2 Gene
Figure 1: Schematic of the MECP2 Gene
The structure of MECP2 is shown, along with the primary (E1) and minor, alternate (E2) transcripts. The insertion site is downstream of the start codons for both transcripts, resulting in truncation of both proteins and inactivation of the methyl binding domain
Advantages
- Human relevance: iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses.
- Reproducible research: Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance from every lot of iCell Microglia (>80% TREM2+, IBA1+, CX3CR1+).
- Rapid results: iCell Microglia are fully differentiated cells (not precursors) allowing for more experiments in less time.
- Disease lines available: TREM2, SNCA and MeCP2 engineered mutants and isogenic control.
Publications
iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases Abud EM, Ramirez RN, Martinez ES, Healy LM, Nguyen CHH, Newman SA, Yeromin AV, Scarfone VM, Marsh SE, Fimbres C, Caraway CA, Fote GM, Madany AM, Agrawal A, Kayed R, Gylys KH, Cahalan MD, Cummings BJ, Antel JP, Mortazavi A, Carson MJ, Poon WW, Blurton-Jones M (2017) Neuron. 94(2):278-293 (2017)