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Innervation of muscle fibers by spinal motor neurons is essential for controlling muscle contraction and ultimately coordinated movement. Neurodegenerative diseases, such as ALS and SMA, are a result of the progressive degeneration of motor neurons that leads to an uncoupling of this process. In order to provide researchers with a model system for understanding basic motor neuron biology and to develop treatments for these diseases, FCDI offers iCell® Motor Neurons. iCell Motor Neurons are a highly pure population of human spinal motor neurons derived from induced pluripotent stem (iPS) cells with the following features:
These cells provide a reliable source of human neurons suitable for use within:
Flow cytometry data verify a highly pure (Tuj positive), fully differentiated (Nestin negative) motor neuron (Isl1/2) population.
The cells display typical morphology, developing branched networks within 1 DIV (Panel A), expanding beyond 7 DIV (Panel B) and forming ganglionic structures near 14 DIV (Panel C).
iCell Motor Neurons were stained at 14 DIV for characteristic neuronal and motor neuron markers.
qPCR was performed at 14 DIV to assess the relative expression of neuronal and motor neuron markers within two different lots of iCell Motor Neurons, spinal cord, and iPSCs.
iCell Motor Neurons are amenable to a variety of uses, including co-culture with human skeletal myotubes or plating on multielectrode array (MEA) platforms. Additional suitable applications are: