1536-well Screening Assays with iCell Cardiomyocytes2

1536-well plates are the format of choice for high-throughput screening (HTS). The high density/low well volume necessitates minimal cell and reagent handling and has historically precluded the use of native cells. As such, HTS methodologies have traditionally been target-based efforts against heterologous cell lines expressing one, or at most, a few targets. iCell® Cardiomyocytes2 are changing this paradigm. The shortened workflow and in vivo biology of iCell Cardiomyocytes2 are bringing relevant phenotypic-based screens and the resultant advantages into HTS applications.

With a 4-day workflow and single media change, iCell Cardiomyocytes2 supply an optimal native cell type for cardiac-based discovery and toxicity screens. Easy to follow plating procedures produce an even monolayer of functional cardiomyocytes (Figure 1). In addition, dose response relations provide an excellent assay window (Figure 2) and show congruence with 96-well assays (Table 1).

Together, these qualities are enabling early screening on cells with native behavior and physiology. Contact CDI’s Technical Support for additional information on implementing iCell Cardiomyocytes2 in 1536-well plates.