iCell Cardiomyocytes, 01434, 11713 Serum-Free Kit

iCell Cardiomyocytes, 01434 Serum-Free Kit

Kit Size

Catalog #: R1223
Catalog #: R1225

Cardiomyocytes Serum-Free Kits differentiated from human iPS cells, frozen

From
$1,611.00

iCell Cardiomyocytes, 11713 Serum-Free Kit

Kit Size

Catalog #: R1225
Catalog #: R1225

Cardiomyocytes Serum-Free Kits differentiated from human iPS cells, frozen

From
$1,611.00
Catalog # R2XXX

Product Overview

iCell®Cardiomyocytes and iCell Cardiomyocytes2 from FUJIFILM Cellular Dynamics, provide relevant biology and rapid workflows making them powerful tools for safety, toxicology, and drug discovery research. FCDI offers a full suite of cardiomyocyte media that are all DMEM-based with the same physiological ion concentrations and mature glucose-free energy source. Rigorously designed and quality controlled to work with iCell Cardiomyocytes and Cardiomyocytes2, these media provide researchers the ability to seamlessly switch between serum-containing, serum-free, and albumin-free conditions, enabling acute and chronic studies.

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Performance Data

iCell Cardiomyocytes2 Maintain High Purity, Viability, and Functional Properties Across Platforms When Cultured in iCell Cardiomyocytes Serum-Free Medium (iCSFM), iCell CardioTox Assay Medium (iCTAM) Similar to iCell Cardiomyocytes2 Cultured in iCell Cardiomyocytes Maintenance Medium (iCMM)

(A) High cardiac purity by cTNT flow cytometry, and (B) High viability as indicated by CyQUANT Direct Cell Proliferation Assay (normalized to maximal response per experiment and reported as % iCMM) at end of recommended workflow: D14 cultured in iCMM or iCSFM; D11 cultured in iCTAM. (Purity n=3-6; Viability n= 64-90). (C) Using the xCELLigence CardioRTCA, iCell Cardiomyocytes2 cultured in iCSFM or iCTAM maintained comparable cell index (measure of cell attachment and health) and beat rate (not shown) to those cultured in iCMM (D7 n=24-48; D11 n=9-18). (D) Using the Maestro Pro MEA platform, iCell Cardiomyocytes2 cultured in iCSFM or iCTAM maintained comparable percent total active electrodes (not shown), beat rate, low beat rate irregularity, and field potential duration (FPD corrected to beat rate) to those cultured in iCMM. We recommend iCTAM for up to 4 days as we observed a reduction in beat rate with cells cultured in iCTAM for 7d. (n=24-48)

Figure 1: iCell Cardiomyocytes2 Maintain High Purity, Viability, and Functional Properties Across Platforms When Cultured in iCell Cardiomyocytes Serum-Free Medium (iCSFM), iCell CardioTox Assay Medium (iCTAM) Similar to iCell Cardiomyocytes2 Cultured in iCell Cardiomyocytes Maintenance Medium (iCMM)

Figure 2: iCell Cardiomyocytes2 Maintain Comparable Contractility and Electrophysiology Across Serum-Free Media

iCell Cardiomyocytes2 Maintain Comparable Contractility and Electrophysiology Across Serum-Free Media

Representative traces for (A) Impedance measurements (xCELLigence), (B) Field potential (with T-wave marked) and (C) Action potential (LEAP) traces from Day 7, 4h after media change. Note: In (B) and (C) the cells had been treated with 0.1% DMSO vehicle control.

Characteristics of iCell Cardiomyocytes2 Contractile Parameters Cultured in iCell Cardiomyocytes Serum-free Medium on the FLEXcyte 96 System

(A) Comparison of mean beats are shown of iCell Cardiomyocytes2 cultured in iCell Cardiomyocytes Serum-free Medium (iCSFM) and serum containing iCell Cardiomyocytes Maintenance Medium (iCMM) 24 hours after medium switch. (B) A bar graph showing contractile parameters (amplitude, beat rate, beat duration, area under curve, upstroke and downstroke slopes) of iCell Cardiomyocytes² cultured in iCell Cardiomyocytes Serum-free Medium after 24 hours. Data is normalized to iCell Cardiomyocytes² cultured in iCell Cardiomyocytes Maintenance Medium. (Data kindly provided by Innovitro and Nanion Technologies)

Figure 3: Characteristics of iCell Cardiomyocytes2 Contractile Parameters Cultured in iCell Cardiomyocytes Serum-free Medium on the FLEXcyte 96 System

Figure 4: Comparative LEAP Traces of Cardiomyocyte Media Cultured Cells

Comparative LEAP Traces of Cardiomyocyte Media Cultured Cells

Representative LEAP traces recorded from iCell Cardiomyocytes2 treated with DMSO (control) or Drug (Nifedipine (50nM) top panels, E4031 (30nM), bottom panel) when cultured in iCMM (left), iCSFM (middle) or iCTAM (right). iCell Cardiomyocytes2 were thawed and plated according to User's Guide and cultured until DIV7 in iCMM. On DIV7 some of the wells were washed and fed with iCSFM (for 4 hours and then dosed) or iCTAM (cultured until DIV11 and then dosed).