iCell Cardiomyocytes², 01434

iCell Cardiomyocytes², 01434

Kit Size

Catalog #: R1059
Catalog #: R1220
Catalog #: R1017

Cells Only

Catalog #: C1058
Catalog #: C1016
Catalog #: C1016

Cardiomyocytes^2 differentiated from human iPS cells, frozen

From
$595.00

iCell Cardiomyocytes² Kit Two Donor Kit, 01434 & 11713

Kit Size

Catalog #: R1219
Catalog #: C1016

Cardiomyocytes^2 differentiated from human iPS cells, frozen

From
$3,295.00
Catalog # GCM201434

Product Overview

iCell® Cardiomyocytes2 are an enhanced version of the extensively validated and predictive iCell Cardiomyocytes product. Derived from induced pluripotent stem cells (iPSCs), iCell Cardiomyocytes2 from FUJIFILM Cellular Dynamics, have been optimized for high-throughput applications.

  • Rapid Results iCell Cardiomyocytes2 are optimized for rapid recovery from cryopreservation and are assay-ready as early as 4 days post-thaw. The cells are provided with complete plating and maintenance medium.
  • Minimal Handling iCell Cardiomyocytes2 require a limited number of hands-on steps, thereby reducing lab time and potential for contamination.
  • Verified Model for Cardiac Safety Assessment iCell Cardiomyocytes2 display intact human cardiac biology with electrophysiological and biochemical responses upon exposure to exogenous agents. The cells are a preferred cellular model for cardiac safety assessment included in the Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative.
  • Fully Supported Optimized protocols for functional assays, which include multielectrode array (MEA), calcium handling, and impedance, enable robust and reproducible measurements of compound-induced effects on human cardiomyocyte physiology. FCDI’s application scientists provide relevant training and timely assistance to support seamless implementation.

Components:

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Technical Docs

Performance Data

Formation of 3D Cardiac Tri-Culture Microtissues

Three-dimensional multi-cellular systems containing iCell Cardiomyocytes or iCell Cardiomyocytes2, iCell Endothelial Cells and primary cardiac fibroblasts have the potential for greater physiological relevance, predictive power, and mechanistic insight than cardiomyocytes alone. For information on 3D systems, see the Application Protocol: Culturing and Assaying Calcium Transients of 3D Cardiac Tri-Culture Microtissues

Figure 1: Structure of 3D Cardiac Tri-culture Microtissues Over Time.
Microtissues were formed containing 5,000 or 10,000 total cells in S-bio 96-well plates. Compact, contracting microtissues were obtained by Day 4. (A) Panel of phase contrast 10X images of 5,000 cell tri-culture spheroids over time using the Incucyte S3. (B) Quantification of 5,000 cell tri-culture microtissue diameter. Each dot represents a microtissue. Mean and SEM are indicated. (C) Comparison of Day 14 microtissue diameter of 5,000 cell tri-culture microtissues with 10,000 cell tri-culture microtissues. Each dot represents a microtissue. Mean and SEM are indicated. (D) H&E staining of Day 14, 3D cardiac tri-culture microtissue formed with 10,000 total cells. Staining shows the absence of a necrotic core.

Figure 2: 3D Cardiac Tri-culture Microtissues Response to Beta Adrenergic Agonist Isoproterenol
(A) Chronotropic response: Control iCell Cardiomyocytes, 11713 only microtissues (CM) and tri-culture microtissues exhibit an increase in beat rate with increasing concentrations of isoproterenol. (B) Inotropic response: Control iCell Cardiomyocytes, 11713 only microtissues (CM) do not increase amplitude with increasing concentrations of isoproterenol. Tri-culture microtissues demonstrate a twofold increase in beat amplitude with increasing concentrations of isoproterenol.

Enhanced Inotropic Response when Plated in Tri-Culture

3D cardiac tri-culture microtissues containing iCell Cardiomyocytes or iCell Cardiomyocytes2, iCell Endothelial Cells and cardiac fibroblasts demonstrated a positive response to the inotropic compound isoproterenol, which is characteristic of mature cardiomyocytes. For information on 3D triculture systems, see the Application Protocol: Culturing and Assaying Calcium Transients of 3D Cardiac Tri-Culture Microtissues

Compatible with High-Sensitivity Detection of Calcium Transients in Tri-Culture

Three-dimensional multi-cellular systems containing iCell Cardiomyocytes or iCell Cardiomyocytes2, iCell Endothelial Cells and cardiac fibroblasts demonstrate enhanced amplitude in calcium transient assays. For information on 3D systems, see the Application Protocol: Culturing and Assaying Calcium Transients of 3D Cardiac Tri-Culture Microtissues

Figure 3: Baseline Calcium Transients in Cardiomyocyte and Tri-culture Microtissues at Day 14
Calcium transients were measured using EarlyTox calcium dye. (A) Representative calcium traces for 5,000 and 10,000 total cell 3D Tri-culture cardiac microtissues. (B) Beat rate is not different between 5,000 or 10,000 cell triculture microtissues and control cardiomyocyte only microtissues. (C) Representative calcium traces for 5,000 cell tri-culture and 5,000 cell cardiomyocytes only microtissues (CM only). (C) Amplitude is significantly higher in triculture microtissue compared to cardiomyocyte only microtissues (CM only) at 5,000 cells, but similar at 10,000 cells

Figure 4: iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel

iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel

Panels A and B show the expected increase in the field potential duration blocking IKr with E-4031. Panels C and D show the expected decrease in the field potential duration blocking the L-type calcium channels with NI. iCell Cardiomyocytes2 were exposed to the indicated compounds at the concentrations listed and the effects quantified ± SEM.

iCell Cardiomyocytes2 Capture Phenotypic Responses across Different Classes of Cardioactive Compounds

Modulating ion channel and GPCR activity alters the spontaneous contractile activity of iCell Cardiomyocytes2. Blocking IKr, ICa-L, and INa with E4031, NI, and INN and stimulating the β-adrenergic pathway with ISO produced the expected effects on the beat waveforms.

Figure 5: iCell Cardiomyocytes2 Capture Phenotypic Responses across Different Classes of Cardioactive Compounds

Figure 6: iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel Block

iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel Block

Panels A and B show the expected increase in the field potential duration blocking IKr with E-4031. Panels C and D show the expected decrease in the field potential duration blocking the L-type calcium channels with NI. iCell Cardiomyocytes2 were exposed to the indicated compounds at the concentrations listed and the effects quantified ± SEM.

Product Highlights

A Multitude of Applications

iCell Cardiomyocytes2 have demonstrated utility for electrophysiological and biochemical assays in toxicology, drug discovery, and basic life science research applications. Contact our Technical Support team for information on using iCell Cardiomyocytes2 in these types of studies.

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