iCell Cardiomyocytes^2, 01434

Kit Size

Catalog #: R1017

Cells Only

Catalog #: C1016
Catalog #: C1016

Cardiomyocytes^2 differentiated from human iPS cells, frozen

From
$1,650.00
Catalog # GCM201434

Product Overview

iCell® Cardiomyocytes2 are an enhanced version of the extensively validated and predictive iCell Cardiomyocytes product. Derived from induced pluripotent stem cells (iPSCs), iCell Cardiomyocytes2 from FUJIFILM Cellular Dynamics, have been optimized for high-throughput applications.

  • Rapid Results iCell Cardiomyocytes2 are optimized for rapid recovery from cryopreservation and are assay-ready as early as 4 days post-thaw. The cells are provided with complete plating and maintenance medium.
  • Minimal Handling iCell Cardiomyocytes2 require a limited number of hands-on steps, thereby reducing lab time and potential for contamination.
  • Verified Model for Cardiac Safety Assessment iCell Cardiomyocytes2 display intact human cardiac biology with electrophysiological and biochemical responses upon exposure to exogenous agents. The cells are a preferred cellular model for cardiac safety assessment included in the Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative.
  • Fully Supported Optimized protocols for functional assays, which include multielectrode array (MEA), calcium handling, and impedance, enable robust and reproducible measurements of compound-induced effects on human cardiomyocyte physiology. FCDI’s application scientists provide relevant training and timely assistance to support seamless implementation.

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Technical Docs

Performance Data

iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel

Panels A and B show the expected increase in the field potential duration blocking IKr with E-4031. Panels C and D show the expected decrease in the field potential duration blocking the L-type calcium channels with NI. iCell Cardiomyocytes2 were exposed to the indicated compounds at the concentrations listed and the effects quantified ± SEM.

Figure 1: iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel

Figure 2: iCell Cardiomyocytes2 Capture Phenotypic Responses across Different Classes of Cardioactive Compounds

iCell Cardiomyocytes2 Capture Phenotypic Responses across Different Classes of Cardioactive Compounds

Modulating ion channel and GPCR activity alters the spontaneous contractile activity of iCell Cardiomyocytes2. Blocking IKr, ICa-L, and INa with E4031, NI, and INN and stimulating the β-adrenergic pathway with ISO produced the expected effects on the beat waveforms.

iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel Block

Panels A and B show the expected increase in the field potential duration blocking IKr with E-4031. Panels C and D show the expected decrease in the field potential duration blocking the L-type calcium channels with NI. iCell Cardiomyocytes2 were exposed to the indicated compounds at the concentrations listed and the effects quantified ± SEM.

Figure 3: iCell Cardiomyocytes2 Provide an Accurate System for Detecting Ion Channel Block

Product Highlights

A Multitude of Applications

iCell Cardiomyocytes2 have demonstrated utility for electrophysiological and biochemical assays in toxicology, drug discovery, and basic life science research applications. Contact our Technical Support team for information on using iCell Cardiomyocytes2 in these types of studies.