iCell GlutaNeurons, 01279

Kit Size

Catalog #: R1061
Catalog #: R1116
Catalog #: R1034
Catalog #: R1034

GlutaNeurons differentiated from human iPS cells, frozen

From
$595.00
Catalog # GGLN01279

Product Overview

Understanding basic neuronal physiology and discovering therapeutics to treat neurological disorders has relied heavily on rodent primary cell cultures and animal models. These common systems have significant drawbacks in terms of biological relevance, reproducibility, and scalability. iCell® GlutaNeurons provide a relevant, excitatory neuronal model that enables researchers to study human neuronal network development and activity through interrogation and manipulation of relevant pathological pathways involved in seizurogenic and neurodegenerative conditions, thereby providing a new and valuable tool for drug discovery, toxicity testing, and basic research.

iCell GlutaNeurons, human glutamatergic-enriched cortical neurons derived from induced pluripotent stem (iPS) cells, display typical physiological characteristics and form functional neuronal networks amenable to examination across a number of commonly used assay techniques. These cells overcome limitations of existing models by providing the following:

  • Fully differentiated, ≥90% pure population of primarily glutamatergic (excitatory) human neurons
  • Long-term viability, demonstrated reproducibility, and availability in commercial quantities
  • Rapid formation of excitatory neural networks and functional synapses
  • Expression of relevant neurological therapeutic targets and pathways
  • Compatible with a wide range of biochemical and cell-based assays including:
    • Cell viability
    • Calcium signaling
    • Neurite outgrowth and retraction
  • Electrophysiological applications:
    • Identification and characterization of network function
    • Higher throughput assessment of compound efficacy for seizure treatment
    • Higher throughput detection of seizurogenic toxicity
  • Ease of use; simply thaw and plate

Components:

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Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

Purity and Gene Expression Analysis of iCell GlutaNeurons

(A) The cells display typical morphology, developing branched networks within 24 hours. (B) Flow cytometry data verify a highly pure, fully differentiated neuronal population. (C) Immunofluorescent labeling identifies the synaptic marker synaptophysin, neuronal marker tuj-1, and nuclei. (D) Single-cell gene expression analysis confirms the high proportion of glutamatergic neurons, which enables the formation of synchronously bursting networks.

Figure 1:  iCell GlutaNeurons Provide a Highly Pure Population of Glutamatergic Cortical Neurons

Figure 2: iCell GlutaNeurons Provide a Relevant Human-Derived Model for Investigating Neurotoxicity and Identifying Neuroprotectants

Neurotoxicity Assay

(A - D) Upon increased exposure to glutamate, iCell GlutaNeurons exhibit changes in their viability as assessed by, but not limited to, LDH release (CytoTox-ONE assay, Promega), reducing potential (RealTime-Glo assay, Promega), ATP presence (CellTiter-Glo assay, Promega), or membrane integrity (CyQUANT assay, Thermo Fisher Scientific), respectively. (E) Glutamate-induced cell death can be ameliorated by inhibition of NMDA (AP5) and AMPA (DNQX) receptors, as assessed by LDH release, highlighting the utility of iCell GlutaNeurons in screening for neuroprotectants

Product Highlights

Acute and long-term testing

 iCell GlutaNeurons remain viable and pure in culture for more than 4 weeks, enabling assessment of synapse formation as well as network development and disruption.

Easy to implement

iCell GlutaNeurons are shipped cryopreserved with optimized media. Simply thaw and use.

Self-assembling networks

The capability to form predominantly excitatory neural networks provide a powerful tool in basic research and drug discovery studies.

Homogenous and reproducible

Commercial quantities of consistent batches ensure reproducible large-scale screens and long-term projects.

Highly pure human cells

Terminally differentiated from human iPS cells, iCell GlutaNeurons provide a uniquely relevant biological model.