iCell GlutaNeurons, 01279

Understanding basic neuronal physiology and discovering therapeutics to treat neurological disorders has relied heavily on rodent primary cell cultures and animal models. These common systems have significant drawbacks in terms of biological relevance, reproducibility, and scalability. iCell^® GlutaNeurons provide a relevant, excitatory neuronal model that enables researchers to study human neuronal network development and activity through interrogation and manipulation of relevant pathological pathways involved in seizurogenic and neurodegenerative conditions, thereby providing a new and valuable tool for drug discovery, toxicity testing, and basic research.

iCell GlutaNeurons, human glutamatergic-enriched cortical neurons derived from induced pluripotent stem (iPS) cells, display typical physiological characteristics and form functional neuronal networks amenable to examination across a number of commonly used assay techniques. These cells overcome limitations of existing models by providing the following:

• Fully differentiated, ≥90% pure population of primarily glutamatergic (excitatory) human neurons
• Long-term viability, demonstrated reproducibility, and availability in commercial quantities
• Rapid formation of excitatory neural networks and functional synapses
• Expression of relevant neurological therapeutic targets and pathways
• Compatible with a wide range of biochemical and cell-based assays including:
  • Cell viability
  • Calcium signaling
  • Neurite outgrowth and retraction
• Electrophysiological applications:
  • Identification and characterization of network function
  • Higher throughput assessment of compound efficacy for seizure treatment
  • Higher throughput detection of seizurogenic toxicity
• Ease of use; simply thaw and plate