iCell Macrophages Kit, 01279

Macrophages are a type of leukocyte responsible for mediating inflammatory and immune responses. As part of the innate immune response system, these cells ingest unwanted material from the body by the process of phagocytosis, thereby removing cell debris, bacteria, and other foreign matter. Additionally, they can release cytokines that in turn signal other cells to mount inflammatory or anti-inflammatory responses. Macrophages are found as specialized subtypes in multiple organs within the body (e.g. Kupffer cells in the liver) where they have specific functions, such as mediating responses to toxic agents. Because macrophages have been implicated recently as playing a key role in tumorigenesis and evading the immune system response, targeting tumor-associated macrophages holds significant promise for therapeutic development efforts. FCDI’s iCell® Macrophages are human iPS cell-derived macrophages. They provide a simple, reproducible, and biologically relevant source of macrophages to model and interrogate host defense and immunity, as well as pathogenesis of inflammatory and degenerative disease. Benefits include:

• >90% pure population of human CD68+ macrophages
• Characteristic macrophage morphology
• Endotoxin (LPS)-mediated secretion of cytokines (IL-6, TNFα)
• Phagocytosis of albumin and zymosan bacterial particles
• Industrial quantities in a cryopreserved format

Model Disease, Interrogate Biology, Develop Therapies

Assayed as a pure culture of macrophages or a co-culture with CDI’s other cell types, iCell Macrophages provide access to relevant human biology that can be used to conduct:

• Immune and inflammatory response assays
• Infectious disease modeling
• Toxicity testing

Advantages

• Human pedigree:
  iCell Macrophages are differentiated from human iPS cells and exhibit functional characteristics similar to native human macrophages.
• Homogenous and reproducible:
  iCell Macrophages are highly pure, providing biologically relevant and reproducible results.
• Acute and long-term testing capabilities:
  iCell Macrophages provide consistency for co-culture studies and can be grown with cells of the same or a different genotype.
• Easy to implement:
  iCell Macrophage are shipped cryopreserved. Simply thaw and use.