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iCell Motor Neurons, 01279
Product Overview
Innervation of muscle fibers by spinal motor neurons is essential for controlling muscle contraction and ultimately coordinated movement. Neurodegenerative diseases, such as ALS and SMA, are a result of the progressive degeneration of motor neurons that leads to an uncoupling of this process. In order to provide researchers with a model system for understanding basic motor neuron biology and to develop treatments for these diseases, FCDI offers iCell® Motor Neurons. iCell Motor Neurons are a highly pure population of human spinal motor neurons derived from induced pluripotent stem (iPS) cells with the following features:
- Fully differentiated, ≥90% (β-III tubulin) pure population of primarily cholinergic human neurons (≥70% Isl1/2 at 14 days in vitro)
- Display typical neuronal morphology with branching neurites and remain viable for an extended culture period (≥14 days)
- Express markers characteristic of motor neurons
- Available in commercial quantities
- Easy to use: Simply thaw and plate
These cells provide a reliable source of human neurons suitable for use within:
- Basic and translational research: Studying neurodegenerative diseases like ALS & SMA, myelination/demyelination, and neurogenesis
- Drug discovery: For diseases such as ALS and SMA
- Bioengineering: Development of neuromuscular junction models
- Applied markets: BoNT testing
- Toxicology and safety pharma: For testing chemical warfare agents, effects of chemical pesticides, etc.
Components:
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Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
USER DOCS
Biosafety Document
iCell Motor Neurons Biosafety Document
Datasheet
iCell Motor Neurons
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Germany
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - North America
Safety Data Sheet
iCell Neural Base Medium 1 Safety Data Sheet - Europe
Safety Data Sheet
iCell Neural Base Medium 1 Safety Data Sheet - North America
Safety Data Sheet
iCell Neural Supplement A Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement A Safety Data Sheet - Europe
Safety Data Sheet
iCell Neural Supplement A Safety Data Sheet - North America
User's Guides
iCell Motor Neurons
Performance Data
iCell Motor Neurons are High Purity
Flow cytometry data verify a highly pure (Tuj positive), fully differentiated (Nestin negative) motor neuron (Isl1/2) population.
Figure 1: Flow Cytometry Analysis
Figure 2: Morphology
Expected Motor Neuron Morphology
The cells display typical morphology, developing branched networks within 1 DIV (Panel A), expanding beyond 7 DIV (Panel B) and forming ganglionic structures near 14 DIV (Panel C).
Immunocytochemical Analysis Shows Expected Neuronal Markers
iCell Motor Neurons were stained at 14 DIV for characteristic neuronal and motor neuron markers.
Figure 3: Immunocytochemical Analysis
Figure 4: Gene Expression Analysis
Gene Expression Analysis of iCell Motor Neurons: Lot to Lot Consistency
qPCR was performed at 14 DIV to assess the relative expression of neuronal and motor neuron markers within two different lots of iCell Motor Neurons, spinal cord, and iPSCs.
Product Highlights
Diverse Applications
iCell Motor Neurons are amenable to a variety of uses, including co-culture with human skeletal myotubes or plating on multielectrode array (MEA) platforms. Additional suitable applications are:
Cell-based Assays
- Cell viability
- Calcium signaling
- Metabolic assays
- Mitophagy
- Neurite outgrowth and retraction
Electrophysiological Applications
- Conventional patch clamp recording
- MEA recording
Advantages of iCell Motor Neurons
- Human relevance: iCell Motor Neurons recapitulate human spinal motor neurons biology and functionality.
- Reproducible research: High purity (>70% ISL1+ at 14 DIV) and rigorous quality control ensure consistent mechanistic studies of neurodegenerative diseases.
- Rapid results: Get results faster with optimized media and fully differentiated cryopreserved neurons, not precursors. Just thaw, plate, and assay.
Publications
Modeling the Pathogenesis of X-Linked Distal Hereditary Motor Neuropathy Using Patient-Derived iPSCs Perez-Siles G, Cutrupi A, Ellis M, Kuriakose J, La Fontaine S, Mao D, Uesugi M, Takata RI, Speck-Martins CE, Nicholson G, Kennerson ML (2020) Dis Model Mech. 13(2) (2020)
Engineering a 3D Functional Human Peripheral Nerve in vitro Using the Nerve-on-a-Chip Platform Sharma AD, McCoy L, Jacobs E, Willey H, Behn JQ, Nguyen H, Bolon B, Curley JL, Moore MJ (2019) Sci Rep. 9(1):8921. (2019)