How mature are iCell Cardiomyocytes?
Since maturity is a relatively subjective term dependent upon a variety of characteristics, the developmental classification of iCell® Cardiomyocytes is difficult. These terminally differentiated cells exhibit spontaneous beat activity, which aligns with an immature, neonatal electrophysiological state, but their sarcomeric organization, metabolic profile, cell-cell communication, and MLC2v expression are characteristics that align with more advanced developmental stages. For the sake of simplicity, we tend to classify iCell Cardiomyocytes as post-neonatal. Together these characteristics provide a functional human cardiomyocyte model.
For additional characterization details, refer to the peer-reviewed publications below regarding basic research applications, disease modeling, and toxicity testing.
- Ma et al. 2011. High Purity Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Electrophysiological Properties of Action Potentials and Ionic Currents.
- Lee et al. 2012. Simultaneous Voltage and Calcium Mapping of Genetically Purified Human Induced Pluripotent Stem Cell derived Cardiac Myocytes Monolayers.
- Puppala et al. 2013. Comparative Gene Expression Profiling in Human-Induced Pluripotent Stem Cell-Derived Cardiocytes and Human and Cynomolgus Heart Tissue.
- Rana et al. 2012. Characterization of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Bioenergetics and Utilization in Safety Screening.