Can I model hypertrophy with your cardiomyocytes?
iCell® Cardiomyocytes recapitulate numerous properties, including hypertrophic response, of native cardiac myocytes. For modeling hypertrophy, you can plate iCell Cardiomyocytes in iCell Cardiomyocytes Plating Medium and maintain the cells in iCell Cardiomyocytes Maintenance Medium, but you will need to switch to the serum-free supplemented William’s E (SWE) medium before your assay.
We have reproducibly demonstrated a hypertrophic response, induced by endothelin-1 (ET-1), in Carlson et al. (2013) using ELISA, flow cytometry, high content analysis, and qRT-PCR methods. Our hypertrophy-specific Application Protocols for these methods use fibronectin for the extracellular matrix (ECM), Plating Medium for thawing and plating cells, supplemented William’s E (SWE) medium for maintaining cells, ET-1 for inducing the hypertrophic response, and B-type natriuretic peptide (BNP) or natriuretic peptide precursor B (NPPB) response detection after the 18 hour incubation.
For more details about the materials required for a specific method, see the Required Equipment and Consumables section in our Application Protocols:
- Modeling Cardiac Hypertrophy by ELISA
- Modeling Cardiac Hypertrophy by Flow Cytometry
- Modeling Cardiac Hypertrophy by High Content Analysis
- Modeling Cardiac Hypertrophy by qRT-PCR
For more details about modeling hypertrophy, please contact Technical Support.
- iCell Cardiomyocytes User's Guide
- Modeling Cardiac Hypertrophy Application Protocols
- Application Protocols: Modeling Cardiac Hypertrophy
- Carlson et al (2013) Phenotypic screening with human iPS cell-derived cardiomyocytes: HTS-compatible assays for interrogating cardiac hypertrophy.
- Aggarwal et al (2014) RNA expression profiling of human iPSC-derived cardiomyocytes in a cardiac hypertrophy model.