What protocol conditions and steps do you recommend for disassociating and harvesting iCell Cardiomyocytes?

We recommend that you culture iCell® Cardiomyocytes for 7 days as described in the User's Guide prior to enzymatic dissociation with 0.1% trypsin described in the Application Protocol. For pre-plated cultures, we recommend 750,000 plated cells per well in 6-well plates (~78,000 cells/cm2).

Reference:

  1. iCell Cardiomyocytes User's Guide
  2. Application Protocol: Dissociating Cardiomyocytes >by Enzymatic Treatment