Which protocol should I use for immunofluorescent labeling of GABANeurons?

Immunofluorescent labeling is a straightforward technique for assessing the presence and the subcellular localization of an antigen or a protein. Our Application Protocol has demonstrated utility in detecting the presence of microtubule-associated protein 2 (MAP2B), γ-aminobutyric acid (GABA), vesicular GABA transporter (vGAT), vesicular glutamate transporter 2 (vGLUT2), and β-tubulin, class III (tuj-1). This protocol has also shown usefulness in assessing the lack of nestin or glial fibrillary acid protein (GFAP) expression on iCell® GABANeurons.

For more details about neural markers, please see Solution 00000078K, below.

Reference:

  1. Application Protocol: Immunofluorescent Labeling