Can I use centrifugation to concentrate cardiomyocytes before plating?

Yes, but centrifugation is not a necessary step. iCell® Cardiomyocytes can tolerate centrifugation at 180 x g for 5 minutes to pellet the cells. If you have a higher density requirement, we recommend you use a smaller volume of iCell Cardiomyocytes Plating Medium for the initial thaw, but be sure to use at least 5 ml. If you choose centrifugation, you may lose some cells, and it becomes important to count cells afterwards and use that number to calculate the plating volume. If you still have cell clumps after centrifugation, gently mix the cells by manual pipetting.

For more details about mixing the cell suspension by manual pipetting, see Solution 00000073E, below.

Reference:

  1. iCell Cardiomyocytes User's Guide