What plating density should I use for GABANeurons?
You can plate iCell® GABANeurons at various densities to accommodate different application needs. We recommend starting with 125,000 viable cells/cm2 for most cell-based assays. However, the optimal density of iCell GABANeurons per unit of surface area can be assay-dependent and should be determined empirically based on the intended use.
|Culture Vessel||Surface Area |
|Plating Volume |
|Cell Number |
(1.25 x 105 cells/cm2)
|6-well Cell Culture Plate||9.6||3||1,200 x 103|
|96-well Cell Culture Plate||0.32||0.2||40 x 103|
|384-well Cell Culture Plate||0.06||0.04||7.5 x 103|
|1536-well Cell Culture Plate||0.025||0.005||3 x 103|
|All volumes and measures are per well.|