Which extracellular matrix (ECM) do you recommend for your cardiomyocytes?

iCell® Cardiomyocytes are compatible with a variety of ECMs. For standard culture, we recommend coating culture surfaces with a sterile, 0.1% (w/v) gelatin solution, which can be stored stably for up to 3 months at room temperature. For more details about preparing the gelatin solution, see our User’s Guide.

We also have tested other common ECMs, including fibronectin and collagen (with reconstituted powder or pre-coated plate options), Geltrex Matrix (Thermo Fisher Scientific), and Matrigel Matrix (Corning).

The recommended ECM may be assay specific. For our cardiac hypertrophy assays, we recommend fibronectin (see Solution 00000050B, below) because gelatin has been shown to bind endothelin-1 and interfere with the hypertrophic response (see Solution 00000050A, below). For applications using glass coverslips (e.g. manual patch clamp or immunofluorescence), we recommended fibronectin. For more details about assay-specific recommendations, see our Application Protocols.

References:

  1. iCell Cardiomyocytes User's Guide
  2. iCell Cardiomyocytes Application Protocols