Which extracellular matrix (ECM) and coating method do you recommend for culturing GABANeurons?

ECM coating recommendations for iCell® GABANeurons include our preferred manual double-coating method with a fresh base layer of poly-L-ornithine (PLO) and a fresh top coat of laminin or use of a pre-coated poly-D-lysine (PDL) plate with a fresh coat of laminin according to our User’s Guide. These ECM options provide the amino acid base required for adherence and producing typical neurite outgrowth. The following ECMs are not recommended for use with iCell GABANeurons: gelatin, fibronectin, Matrigel, or collagen.

For more details about ECMs for use with glass plates or coverslips, see Solutions 00000083D and 00000083E, below.

For more details about using pre-coated PDL plates, see Solution 00000083B, below.

References:

  1. iCell GABANeurons User's Guide
  2. Application Protocol: Plating into 1536-well Cell Culture Plates
  3. Application Protocol: Measuring Neuronal Activity: Extracellular Single-unit Recordings on the Maestro Multielectrode Array