Is it necessary to completely remove the laminin from plates before adding GABANeurons?

No, a low concentration of laminin (1 μg/ml) can remain in iCell® GABANeurons culture maintenance to support neurite health (see our MEA Application Protocol or 1536-well Application Protocol).

References:

  1. iCell GABANeurons User's Guide
  2. Application Protocol: Plating into 1536-well Cell Culture Plates Application Protocol
  3. Application Protocol: Multielectrode Array System Application Protocol