Can I culture your GABANeurons with or without serum or an extracellular matrix (ECM)?

iCell® GABANeurons can be cultured with or without serum (the provided medium, iCell Neural Base Medium 1, does not contain serum), but the cells do require an ECM. We recommend using poly-L-ornithine (PLO), poly-D-lysine (PDL), or polyethyleneimine (PEI) plates as appropriate, coated with fresh laminin. Cells do not adhere easily to gelatin, fibronectin, collagen or Matrigel matrices.

For more details about ECMs and culture vessel types, please see Solution 00000083D, below.

References:

  1. iCell GABANeurons User's Guide
  2. Application Protocol; iCell GABANeurons - Plating into 1536-well Cell Culture Plates
  3. Application Protocol: iCell GABANeurons - Measuring Neuronal Activity: Extracellular Single-unit Recordings on the Maestro Multielectrode Array