What feeding schedule do you recommend for cardiomyocytes?

We recommend feeding iCell® Cardiomyocytes every 48 hours post-plating. Perform 100% medium change from iCell Cardiomyocytes Plating Medium to iCell Cardiomyocytes Maintenance Medium on day 2 post-plating and then perform 100% medium change using Maintenance Medium every 48 hours thereafter.

Tips

  • Making the first medium change before 48 hours may result in lower plating efficiency.
  • Medium change timing can affect beat rate.
  • Avoid repeated warming/cooling of the medium stock bottle.
  • Dispense the Maintenance Medium into smaller aliquots suitable for each feed (see related solution, below).

Change the medium at least 4 hours before performing experiments; avoid a medium change immediately before experiments.

Reference:

  1. iCell Cardiomyocytes User's Guide