How can I avoid having to feed cardiomyocytes over the weekend?

Perform 100% medium change late on Friday and again as early as possible on Monday. We recommend you do not assay the cells on the Monday after a feed-free weekend as they may need time to recover, especially for beating assays. Also, do not thaw cells on a Friday and keep in iCell Cardiomyocytes Plating Medium over the weekend.