How do you verify the metabolism of your cardiomyocytes?

We have verified oxidative phosphorylation in iCell^® Cardiomyocytes using an XF96 Extracellular Flux Analyzer (Seahorse Biosciences). In addition, Rana et al. (2012) tested for oxidative phosphorylation and glycolysis by measuring real-time oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) in iCell Cardiomyocytes using a Seahorse Biosciences instrument.

Reference:

1. Rana et al. (2012) Characterization of Human-induced Pluripotent Stem Cell–derived Cardiomyocytes: Bioenergetics and Utilization in Safety Screening