What cell density do you use for neurite outgrowth analysis of GABANeurons? How do you evaluate the neurite outgrowth of your GABANeurons?

We perform neurite outgrowth analysis for iCell® GABANeurons at a density of 15,000 cells/well in a 96-well plate according to our Application Protocol. For analysis of fixed cells, we use a βIII-tubulin antibody; for live cells, we use a calcein AM viability stain; for staining nuclei, we use a Hoechst 33342 stain. Tice et al. and Sherman et al. assessed the effect of multiple compounds on the neurite outgrowth.

References:

  1. Application Protocol: Immunofluorescent Labeling
  2. Application Protocol: Neurite Outgrowth Application Protocol
  3. Poster Presentation: Tice et al. Using Human-Induced Pluripotent Stem Cells (iPSC) in High-Content Screening (HCS) to Prioritize Chemicals for Developmental Neurotoxicity Testing
  4. Poster Presentation: Sherman et al. High content screen for compounds that modulate neurite outgrowth and retraction using human induced pluripotent stem cell derived neurons