Do cardiomyocyte media contain antibiotics or antimitotics? Can I add antibiotics to these media and will the addition affect my assays?

No, iCell® Cardiomyocytes Plating and Maintenance Medium used with iCell Cardiomyocytes and iCell Cardiomyocytes2 are antibiotic- and antimitotic-free. Yes, you can add antibiotics to your culture as a preventative measure against inadvertent bacterial contamination. For certain applications (MEA, Seahorse, others) we have added 25 µg/ml gentamicin (ThermoFisher Scientific, Cat. No. 15750-060) as early as day 2 post-thaw to iCell Cardiomyocytes when switching from Plating Medium to Maintenance Medium. Alternatively, iCell Cardiomyocytes will tolerate 1X penicillin-streptomycin (ThermoFisher Scientific, Cat. No. 15140122). For iCell Cardiomyocytes2, you can add an antibiotic when switching to Maintenance Medium 4 hours post thaw. While we have not performed thorough assessments of functional impacts of these antibiotics on the cells, we have qualitatively observed no significant impact.


  1. iCell Cardiomyocytes2 User Guide