We recommend feeding iCell® DopaNeurons on day 2 post-thaw and every 2 to 3 days thereafter. All medium changes should be a 75 - 100% exchange to fresh Maintenance Medium from an aliquot warmed to room temperature. Be sure to use careful aspiration practices that don’t touch the plated cells. To minimize degradation of medium components, avoid repeated warming and cooling of the stock bottle.

Reference:

  1. iCell DopaNeurons User's Guide