What is the recommended feeding schedule for hepatocytes?

We recommend feeding iCell® Hepatocytes 2.0 with iCell Hepatocytes Plating Medium at 4 hours post-thaw and then daily for 4 days post-thaw. On day 5 post-thaw and every other day thereafter, perform a 100% medium change to iCell Hepatocytes 2.0 Maintenance Medium. For certain drug testing applications, you can change the medium more frequently.


  1. iCell Hepatocytes 2.0 User's Guide