How are the media for hepatocytes prepared? Why are additional components added?

CDI recommends culturing iCell® Hepatocytes 2.0 in RPMI-based plating and maintenance media (not provided) supplemented with iCell Hepatocytes 2.0 Medium Supplement (provided as a component of the iCell Hepatocytes 2.0 Kit, Cat. No. R1027) and several other components (not provided). Media were formulated to maintain purity and function of cells over time in culture.

The Plating Medium consists of the six components listed in the table below; the Maintenance Medium consists of the same components as the Plating Medium except it does not contain oncostatin M.

Component Vendor Catalog Number Stock Solution Final Concentration
RPMI Thermo Fisher Scientific 11875 1X 1X
B-27 Supplement, 50X Thermo Fisher Scientific 17504 50X 1X
Oncostatin M* R&D Systems 295-OM 10 μg/ml in 0.1% BSA in DPBS 20 ng/ml
Dexamethasone Fisher Scientific ICN19456125 5 mM in DMSO 0.1 μM
Gentamycin Thermo Fisher Scientific 15750 50 mg/ml 25 μg/ml
iCell Hepatocytes 2.0 Medium Supplement Cellular Dynamics M1024 50X 1X

For more information about stability and storage recommendations, please see Solution 00000202C, below.

  1. iCell Hepatocytes 2.0 User's Guide