How much media can I prepare from the supplement provided in a hepatocyte kit?

We provide two 3 ml aliquots of iCell® Hepatocytes 2.0 Medium Supplement in each kit, from which you can prepare approximately 300 ml of media. This volume is sufficient to support cell culture for approximately 4 weeks with 75 ml of Plating Medium needed for the first 4 days in culture and 225 ml of Maintenance Medium needed for the duration of culture.


  1. iCell Hepatocytes 2.0 User's Guide