What are the purity and maturity characteristics of your hepatocytes?

iCell® Hepatocytes 2.0 are a >95% pure population of iPSC-derived hepatocytes as based on AAT expression and the absence of iPSC markers. These cells show a mixed gene expression profile with high albumin (ALB) expression levels (≥80% pure as determined by albumin staining at day 7 post-plating), which is indicative of mature adult hepatocytes. However, the cells exhibit elevated AFP and CYP3A7 mRNA levels, which is more typical in fetal hepatocytes. Note that a mixed gene expression profile is observed in many iPSC-derived cell types.

See our Application Protocols:

For more details about the morphology of our hepatocytes, see Solution 00000201A, below.


  1. iCell Hepatocytes Data Sheet
  2. iCell Hepatocytes 2.0 User’s Guide
  3. iCell Hepatocytes Immunofluorescent Labeling Application Protocol
  4. Application Protocol: Labeling Hepatic Markers: Alpha-1 Antitrypsin Labeling with Flow Cytometry Analysis
  5. Application Protocol: Modeling 3D Liver Tissue: 3D Hepatocyte Spheroids in Low Attachment Plates