How do I maintain and passage iCell Endothelial Cells?

iCell® Endothelial Cells can be maintained in culture using VascuLife VEGF Medium (Lifeline Cell Technologies) and are guaranteed to maintain their purity, phenotype, and functionality for five passages, when maintained according to the methods specified in our User’s Guide. Typically, these cells double every 1.5 days, and are ready to passage approximately 3 or 4 days after splitting. We have always characterized and used these cells within the first five passages. We have no data to support cultures longer than the specified five-passage time frame or for cryopreserving these cells.


  1. iCell Endothelial Cells User’s Guide