How do iCell Endothelial Cells compare to HUVECs?

iCell® Endothelial Cells, highly dynamic cells that form the inner lining of blood vessels that are derived from human induced pluripotent stem cells, share some characteristics that are similar to human umbilical vein endothelial cells (HUVECs), including similar morphology and similar gene and protein expression of key endothelial markers and angiogenesis-related genes (e.g., CD31, CD105, CD144, ZO-1, and von Willebrand Factor (vWF)). In functional assays, both cell types can be used for capillary-like tube formation in a Matrigel® Extracellular Matrix (Corning), demonstrate uptake of acetylated-low density lipoprotein (Ac-LDL), and show cell migration toward FBS chemoattractant. However, of these two types, only iCell Endothelial Cells Cell demonstrate cell invasion in Matrigel assays. Belair et al (2015) provide additional characterization data for iCell Endothelial Cells in in their peer-reviewed publication about modeling vascular tissue formation.

References:

  1. iCell Endothelial Cells Datasheet
  2. iCell Endothelial Cells User’s Guide
  3. Belair et al (2015) Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells