iCell® Cardiomyocytes2, our second version of cardiomyocyte cells, were developed specifically for use on MEA and xCELLigence platforms and derived from the same iPSC clone, making it genetically identical to the first iCell Cardiomyocytes version. We have shown that there are no significant differences between the first version assayed at 14 days post-thaw and iCell Cardiomyocytes2 assayed at 4 and 7 days post-thaw on platforms that measure impedance and/or field potential duration (FPD) in the presence and absence of compounds. Compared to the first version, iCell Cardiomyocytes2 have a faster post-thaw recovery, forming a stably beating monolayer by day 4.

We expect that application protocols written for iCell Cardiomyocytes should work as written for iCell Cardiomyocytes2, except with a shorter workflow due to an earlier assay time frame. Notable differences from the iCell Cardiomyocytes workflow introduced by iCell Cardiomyocytes2 include:

  • Eliminating the need to factor plating efficiency (PE) into the seeding calculation and enabling use of the cell counts provided on the Certificate of Testing
  • Switching the time for exchange of Plating Medium to Maintenance Medium to 4 hours post-thaw (versus 2 days for iCell Cardiomyocytes)
  • Shortening the time to assay to 4 – 8 days post-thaw (versus 14 days for iCell Cardiomyocytes)
  • Recommended seeding density 156,000 cells/cm2

For application-specific differences, refer to the following references, below. For more about characterization of iCell Cardiomyocytes, see solutions 0000090A0000090B0000090C0000090D, and 0000090E.

References:

  1. iCell Cardiomyocytes2 User Guide
  2. Applicarion Protocol: Measuring Cardiac Activity: Impedance and Extracellular Field Potential Detection with xCELLigence RTCA CardioECR System
  3. Application Protocol:  Measuring Cardiac Activity: Impedance Detection with xCELLigence RTCA Cardio System
  4. Application Protocol:Measuring Cardiac Activity: Intracellular Calcium Flux Detection with FDSS/μCELL
  5. Application Protocol: Measuring Cardiac Electrical Activity: Field Potential Detection on the Maestro Multielectrode Array
  6. Ma et al (2011) High Purity Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Electrophysiological Properties of Action Potentials and Ionic Currents