Have you used optical methods to detect the stimulated responses in membrane potential or voltage-sensitive dyes (VSDs) in iCell Cardiomyocytes?

Although we have not developed a procedure for optical monitoring or stimulation of our iCell® Cardiomyocytes, several of our customers have used a combination of VSD, optical mapping, optogenetic, and FRET methods:

Source Title Customer Method used
Poster Optical Measurements of Electrical Activity from hiPSC Derived Cardiomyocytes Is a Robust and High-throughput Method for Measuring NCE Effects on the Cardiac Action Potential Clyde Biosciences combination of VSDs and microscopy
Poster Transmembrane Potential Measurements in Cardiac 3D Microtissues Derived from Human Stem Cells Clyde Biosciences collaboration with InSphero 3D microtissues
Poster Rapid Intensity Modulation of a Single Light Source Allows Excitation of Voltage Sensitive Dye and Intermittent Activation of Channel Rhodopsin in hiPSC Derived Cardiomyocytes (hiPSC‐CMs) Clyde Biosciences optogenetic stimulation with VSDs and intermittent activation of channel rhodopsin (ChR2)
Poster Determination of Proarrhythmic Effects of Compounds in Human iPSC-derived Cardiomyocytes Using FDSS/uCell Imagining Platform Merck Ca2+-sensitive fluorescence dyes (Codex ACTOne® and EarlyTox®) and a membrane potential dye (FLIPR MP®)
Poster Drug Discovery and Toxicity Screening in Stem Cell Models Using Optogenetics Q-State Biosciences combination of optogenetics and lentiviral infection in a dye-free, all optical (Optipatch) system
Peer-reviewed publication EAG Domains Regulate LQT Mutant hERG Channels in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Liu et al (2015) Förster resonance energy transfer (FRET) spectroscopy and patch clamping
Peer-reviewed publication Simultaneous Voltage and Calcium Mapping of Genetically Purified Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers Lee et al (2012) fluorescent imaging for electrophysiological mapping of transmembrane potential (di-8-ANEPPS), intracellular calcium concentration (Fura-4F), and excitation propagation measurements

The Hamamatsu FDSS and Molecular Devices FLIPR instruments can also measure ratiometric changes in membrane potential using VSDs as well as calcium oscillations using calcium dyes.

References:

  1. Poster Presentation: Optical Measurements of Electrical Activity from hiPSC Derived Cardiomyocytes Is a Robust and High-throughput Method for Measuring NCE Effects on the Cardiac Action Potential
  2. Poster Presentation: Transmembrane Potential Measurements in Cardiac 3D Microtissues Derived from Human Stem Cells
  3. Poster Presentation: Rapid Intensity Modulation of a Single Light Source Allows Excitation of Voltage Sensitive Dye and Intermittent Activation of Channel Rhodopsin in hiPSC Derived Cardiomyocytes (hiPSC‐CMs)
  4. Poster Presentation: Determination of Proarrhythmic Effects of Compounds in Human iPSC-derived Cardiomyocytes Using FDSS/uCell Imagining Platform
  5. Poster Presentation: Drug Discovery and Toxicity Screening in Stem Cell Models Using Optogenetics
  6. Liu et al. (2015) EAG Domains Regulate LQT Mutant hERG Channels in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
  7. Lee et al. (2012) Simultaneous Voltage and Calcium Mapping of Genetically Purified Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers