Which protocol do you recommend for harvesting and preparing your hepatocytes for flow cytometry analysis?

iCell® Hepatocytes 2.0 can be dissociated and then stained for flow cytometry analysis. Our Application Protocol demonstrates labeling of alpha-1-antitrypsin in iCell Hepatocytes 2.0 and can serve as a starting point for labeling other hepatic proteins for flow cytometry analysis. Antibody dilution and incubation conditions may need to be optimized for your specific antibodies and target proteins.

References:

  1. iCell Hepatocytes User's Guide
  2. Application Protocol: Labeling Hepatic Markers: Alpha-1 Antitrypsin Labeling with Flow Cytometry Analysis