Which protocol do you recommend for harvesting and preparing your hepatocytes for flow cytometry analysis?

iCell® Hepatocytes 2.0 can be dissociated and then stained for flow cytometry analysis. Our Application Protocol demonstrates labeling of alpha-1-antitrypsin in iCell Hepatocytes 2.0 and can serve as a starting point for labeling other hepatic proteins for flow cytometry analysis. Antibody dilution and incubation conditions may need to be optimized for your specific antibodies and target proteins.


  1. iCell Hepatocytes User's Guide
  2. Application Protocol: Labeling Hepatic Markers: Alpha-1 Antitrypsin Labeling with Flow Cytometry Analysis